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README.md
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dtype: int32
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- name: usage
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dtype: string
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- name: is_grayscale
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dtype: bool
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splits:
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- name: train
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num_bytes: 64503513
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num_examples: 670
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- name: stage1_test
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num_bytes: 9680318
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num_examples: 65
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- name: stage2_test
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num_bytes: 13367920
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num_examples: 106
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download_size: 87548608
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dataset_size: 87551751
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configs:
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- config_name: default
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data_files:
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- split: stage2_test
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path: data/stage2_test-*
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---
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license: cc0-1.0
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pretty_name: 2018 Data Science Bowl (BBBC038) - Nuclei Segmentation
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task_categories:
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- image-segmentation
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tags:
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- medical
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- biomedical
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- microscopy
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- histopathology
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- fluorescence
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- nuclei
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- cell-segmentation
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- bbbc038
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size_categories:
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- n<1K
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configs:
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- config_name: default
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data_files:
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- split: stage2_test
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path: data/stage2_test-*
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---
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# 2018 Data Science Bowl (BBBC038) - Nuclei Segmentation
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2D light-microscopy **cell-nucleus segmentation** assembled across many imaging
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experiments (humans, mice, flies; 22 cell types, 15 resolutions, 30+ experiments).
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The collection deliberately spans **multiple modalities**: fluorescence
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(DAPI / Hoechst), brightfield **H&E histopathology**, and other brightfield -
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making it a standard cross-modality nuclei-segmentation benchmark.
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This is the official **BBBC038v1** release (Broad Bioimage Benchmark Collection),
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the same data used in the Kaggle 2018 Data Science Bowl. **License: CC0 / public
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domain.**
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## Contents & splits
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| Split | Images | Nuclei | Ground-truth source |
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|-------|-------:|-------:|---------------------|
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| `train` (stage1_train) | 670 | 29,461 | native per-nucleus PNG instance masks |
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| `stage1_test` (stage1_test) | 65 | 4,152 | RLE in `stage1_solution.csv` (post-competition) |
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| `stage2_test` (stage2_test) | 106 | 3,716 | RLE in `stage2_solution_final.csv` (post-competition) |
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| **Total** | **841** | **37,329** | |
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**Faithful-naming notes**
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- Most papers cite "DSB2018" = `stage1_train` (670) only, since that is the only
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split distributing *native* instance masks. This repo ships the **full 3-stage**
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set; the test-stage GT was decoded from the official solution-CSV RLE.
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- The raw `stage2_test_final` archive contains ~3,019 images, but only **106 are
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scored** - the rest are intentional **decoys** flagged `Usage=Ignored`. **Only
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the 106 scored images are included here.**
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## Ground truth
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`mask` is a **binary semantic** nucleus mask (mode `L`, values `{0, 255}`): the
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**union of all per-nucleus instances**. For `train` it is the union of the native
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per-nucleus PNG masks; for the test splits it is the union of the RLE-decoded
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nuclei. The RLE decoder was validated against the native train masks
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(pixel agreement = 1.000000). The original per-nucleus **instance** masks remain
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available at [BBBC038](https://bbbc.broadinstitute.org/BBBC038) for
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instance-segmentation use.
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## Columns
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| Column | Type | Notes |
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|--------|------|-------|
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| `image_id` | string | source hash id |
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| `image` | Image | RGB (RGBA fluorescence normalized to RGB) |
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| `mask` | Image | binary semantic, `{0,255}` |
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| `split` | string | `stage1_train` / `stage1_test` / `stage2_test` |
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| `num_nuclei` | int32 | nuclei in this image |
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| `height`,`width` | int32 | image dimensions |
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| `usage` | string | `null` (train) / `Public` (s1) / `Private` (s2) |
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| `is_grayscale` | bool | derived (R==G==B): fluorescence/brightfield vs H&E color |
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`metadata.xlsx` (repo root) is the official 43-row per-experiment provenance table
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(cell type, stain, SNR, resolution).
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## Provenance, overlap & integrity
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- **Provenance:** official BBBC038v1 (Broad Institute), CC0. Counts reconcile with
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the paper (670 / 65 / 106).
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- **Overlap (leakage hazards):** a small fraction of images overlap **BBBC039**.
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The H&E subset shares source-level (TCGA-derived) lineage with H&E nuclei sets
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such as MoNuSeg / PanNuke, though no individually-confirmed shared images.
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- **Curated collection:** assembled from 30+ independent experiments / donor labs.
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## Citation
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Caicedo, J.C., Goodman, A., Karhohs, K.W., et al. *Nucleus segmentation across
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imaging experiments: the 2018 Data Science Bowl.* **Nature Methods** 16(12),
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1247-1253 (2019). doi:10.1038/s41592-019-0612-7
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