Update README.md

README.md

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----
-license: apache-2.0
----
+---
+license: mit
+task_categories:
+  - tabular-classification
+language:
+  - en
+tags:
+  - biology
+  - genomics
+  - base-editing
+  - CRISPR
+  - rare-disease
+  - STXBP1
+  - neurology
+pretty_name: STXBP1 Base Editing Mouse Model Compatibility Sweep
+size_categories:
+  - 100M<n<1B
+---
+
+# STXBP1 Base Editing Parameter Sweep
+
+**654 million parameter combinations analyzed for 171 pathogenic STXBP1 variants**
+
+## Overview
+
+This dataset contains exhaustive parameter optimization results for base editing guide design targeting STXBP1 mutations. It identifies which variants can be studied in **wild-type mouse models** versus those requiring humanized mice.
+
+## 🏆 Perfect Score Variants (100/100)
+
+| Variant | cDNA | Editor | WT Mouse Compatible |
+|---------|------|--------|---------------------|
+| **E53X** | c.157G>T | Prime | ✅ YES |
+| **A297S** | c.889G>T | Prime | ✅ YES |
+| **E302X** | c.904G>T | Prime | ✅ YES |
+| **M443R** | c.1328T>G | Prime | ✅ YES |
+| **L446F** | c.1336C>T | CBE | ✅ YES |
+
+## Dataset Statistics
+
+| Metric | Value |
+|--------|-------|
+| Total analyses | 654,595,200 |
+| Variants | 171 SNVs |
+| Parameter combos/variant | 3,850,560 |
+| Compatible variants | ~95 |
+| Compute time | 35 minutes |
+| Hardware | NVIDIA GH200 (ARM64 + H100) |
+
+## Files
+
+- **`stxbp1_ULTIMATE_best.json`** - Optimal parameters for each variant (recommended)
+- **`stxbp1_ULTIMATE_sweep.csv.gz`** - All 654M analyses (~5GB compressed)
+
+## Parameter Space
+
+| Parameter | Range | Values |
+|-----------|-------|--------|
+| Scan radius | 10-200 bp | 191 |
+| Context radius | 5-100 bp | 96 |
+| Edit window | (1,1) to (20,20) | 210 |
+
+## Scoring System (0-100)
+
+- **Context identity** (40 pts): Human-mouse sequence conservation
+- **Guide quality** (30 pts): Protospacer mismatch penalty
+- **Edit window** (20 pts): Target base in optimal editing positions
+- **WT compatible** (10 pts): Can use wild-type mouse
+
+## Editor Types
+
+| Editor | Change | Count | Example |
+|--------|--------|-------|---------|
+| ABE | A→G | 32 | ABE8e |
+| CBE | C→T | 50 | BE4max |
+| Prime | Any | 82 | PE2/PE3 |
+
+## Usage
+
+```python
+import json
+
+# Load optimal configurations
+with open('stxbp1_ULTIMATE_best.json') as f:
+    best = json.load(f)
+
+# Get K196X optimal parameters
+k196x = best['K196X']
+print(f"Score: {k196x['score']}")
+print(f"Editor: {k196x['edit_type']}")
+print(f"Compatible: {k196x['compatible']}")
+```
+
+## Query the Full CSV
+
+```python
+import duckdb
+
+# Find all parameter combos for K196X scoring > 90
+results = duckdb.query("""
+    SELECT * FROM 'stxbp1_ULTIMATE_sweep.csv.gz'
+    WHERE label = 'K196X' AND score > 90
+    ORDER BY score DESC
+    LIMIT 100
+""").df()
+```
+
+## Reference Sequences
+
+- Human: NM_001032221.6 (1,785 bp, 594 aa)
+- Mouse: NM_009295 (1,785 bp, 594 aa)
+- Overall identity: 90.9%
+
+## Interactive Tool
+
+Test individual variants: [STXBP1 Base Editing Verifier](https://huggingface.co/spaces/SkyWhal3/stxbp1_human_to_mouse_base-editing_context_verifier)
+
+## Citation
+
+```bibtex
+@dataset{stxbp1_base_editing_sweep_2026,
+  author = {Freygang, Adam},
+  title = {STXBP1 Base Editing Mouse Model Compatibility Parameter Sweep},
+  year = {2026},
+  publisher = {HuggingFace},
+  url = {https://huggingface.co/datasets/SkyWhal3/stxbp1-base-editing-sweep}
+}
+```
+
+## License
+
+MIT
+
+## Acknowledgments
+
+- STXBP1 Foundation
+- Lambda Cloud (compute)
+- STXBP1-ARIA Project
+
+---
+*For research use. Not medical advice.*